HemogloBind™ Hemoglobin Depletion From Hemolyzed Serum/Plasma

HemogloBind2

– Has a high degree of specificity for hemoglobin binding up to 10 mg/ml
– Applications in blood substitutes, enzyme recovery and analytical interferences
– Removes hemoglobin from any species including human, sheep, bovine, goat, etc
– Removes hemoglobin from organs, tissues.
– Hemoglobin removal from red blood cell lysate for proteomics and biomarker drug discovery
– The flow through fractions retain their enzymatic and biological activity
– The flow through fractions is compatible with LC-MS, activity based protein profiling and proteomic studies.

HemogloBind™ comes from a class of solid-phase, or surface-based, elastomeric poly-electrolytic surfaces that bind proteins through an empirically derived chemistry combining elements of polymer composition, cross-linking architecture and charge properties. HemogloBind™ is engineered for a high degree of selectivity and does not cross react with most common serum components, making it an excellent tool in numerous applications. These include analytical protocols where optical interference is problematic, such as bilirubin analysis and bulk serum clarification. Hemoglobin variants, as in thalassemia, bind with differential affinity towards HemogloBind™. For purification and/or analysis of hemoglobin, a modest elevation in pH will facilitate desorption of hemoglobin bound to HemogloBind™

Product list:

Cat. Product Quantity Promo Price Order
H0145-05 HemogloBind™ , Hemoglobin Depletion From Hemolyzed Serum/Plasma 5 mL 278 € Order
H0145-15 HemogloBind™ , Hemoglobin Depletion From Hemolyzed Serum/Plasma 15 mL 400 € Order
H0145-50 HemogloBind™ , Hemoglobin Depletion From Hemolyzed Serum/Plasma 50 mL 773 € Order
H0145-15-60P HemogloBind™ , Hemoglobin Depletion From Hemolyzed Serum/Plasma 15 mL (60 Aliquots of 250 ul HemogloBind each) 616 € Order
H0145-50-200P HemogloBind™ , Hemoglobin Depletion From Hemolyzed Serum/Plasma 50 mL (200 Aliquots of 250 ul HemogloBind each) 1188 € Order

AlbuVoid™ – Albumin Depletion Plus Low Abundance Serum Protein Enrichment

Albusorb-and-Albuvoid-bsg-image
– Albumin voids in flow-through >95%, with <30 minute bind/wash/elute protocol
– Low abundance enrichment equivalent or better than hexa-peptides or antibodies
– Disposable, cost-effective, no column regeneration or cross-contamination
– Mild elution maintains tertiary structure and simple transfer to secondary analysis
– The eluted fractions retain their enzymatic and biological activity
– Removes albumin from many species including human, mouse, sheep, bovine, goat, rat, calf, cell culture.
– The eluted fraction is compatible with LC-MS, activity based protein profiling and proteomic studies.

AlbuVoid™ is a albumin depletion reagent kit. It removes albumin from serum and plasma samples while concentrating low abundance, and/or low molecular weight proteins. The AlbuVoid™ protocol uses mild buffers; the protocol conditions are so gentle that native enzyme activity is retained in elution fractions. AlbuVoid™ considerably enhances resolution of proteins below 50 kD, a limitation of alternate enrichment protocols.

AlbuVoid™ does not bind albumin. All other proteins(except albumin) in the sample binds to AlbuVoid™ and then you can elute off all the proteins minus the albumin. Resulting in low abundance serum protein enrichment. It is ideal for applications involving biomarker discovery, enzyme assays, toxicological studies for new drugs, protein profiling using SELDI analysis, protein array pixelation ,1D and 2D gel electrophoresis, LC/MS, and MALDI-TOF MS and cytokines research.

AlbuVoid™ derives from a silica-based library of individual mixed-mode polymeric ligands. The library was designed to facilitate weak binding of proteins, allowing for rapid elution from the matrix without any foreknowledge of the variety of proteins contained in the starting sample. Because of its specific binding properties, AlbuVoid™ depletes high abundance proteins in serum like albumin while improving the resolution of less abundant serum proteins.

Product list:

Cat. Product Quantity Promo Price Order
AVK-05 AlbuVoid™ , Albumin Depletion and Low Abundance Protein Enrichment Kit 5 Preps 356 € Order
AVK-10 AlbuVoid™ , Albumin Depletion and Low Abundance Protein Enrichment Kit 10 Preps 364 € Order
AVK-50 AlbuVoid™ , Albumin Depletion and Low Abundance Protein Enrichment Kit 50 Preps 915 € Order

AlbuSorb™ Albumin Depletion Kit

AlbuSorb2_1

– Removes >90% albumin from 30 mg albumin/ml sample
– Affinity-type equivalence, virtually no cross-reactivity with other proteins
– Bind and elute procedure – simply weigh powder, condition the sample, centrifuge and/or filter, and recover the albumin depleted serum
– Economical new surface technology, not based on affinity chromatography
– Mild conditions maintain tertiary structure of proteins and simple transfer to secondary analysis
– The albumin depleted filtrate retains the enzymatic and biological activity
– Removes albumin from samples such as serum, plasma and from species including human, mouse, sheep, bovine, goat, rat, and calf
– The flow through fraction is compatible with LC-MS, activity based protein profiling and proteomic studies.

Albusorb™ is an albumin depletion reagent supplied as a kit with necessary buffers. With 25ul of starting sample, the yield of albumin depleted serum protein is 0.1-0.2 milligrams. Albusorb™ binds albumin from serum or plasma and is compatible with downstream proteomics methods such as protein array pixelation ,1D and 2D gel electrophoresis, LC/MS, and MALDI-TOF MS. Samples are also prepared for biomarker discovery, toxicological studies for new drugs, enzyme assays, protein profiling using SELDI analysis and cytokines research.

AlbuSorb™ binds to albumin, and serum proteins flow through. Removal of albumin allows enhanced detection of low abundance proteins. AlbuSorb™ comes from a silica-based, separation platform utilizing a new combination of surface microenvironments substituted with low molecular substrates that feature drug-binding motifs. Unlike immuno-affinity, the surfaces utilized are disposable eliminating cycle to cycle variance and cross-contamination.

Product list

Cat. Product Quantity Promo Price Order
A185-6 AlbuSorb™ , Albumin Depletion Kit 6 grams 460 € Order
A185-18 AlbuSorb™ , Albumin Depletion Kit 18 grams 1006 € Order
A185-3 AlbuVoid™ , Albumin Depletion and Low Abundance Protein Enrichment Kit 3 grams 505 € Order

NuGel™ Poly-NHS

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– Non-specific sites are virtually eliminated by a polymer coating
– Stable across a wide pH range 2 – 10
– 1000A, 50µm Silica suitable for LC and batch processes
– Applications include: Enzyme immobilization, separation and purification of biopolymers, immobilization of proteins & ligands – monoclonal antibodies, hormones, peptides, haptens, drugs, etc

Silica has been an industry standard as an advantageous matrix suitable for high performance liquid chromatography. With NuGel™, non-specific sites have been virtually eliminated making it an ideal support for affinity purification. Through a proprietary polymer coating, Silica is crosslinked forming a reactive Poly-Epoxy functionality stable across a wide pH range (pH 2 to 10). From this foundational chemistry, all of the NuGel™ affinity products are derived.

NuGel™ Poly-NHS Technical Data

NuGel™ Poly-NHS is a derivative of NuGel™ polyamino affinity support. This affinity support contains NHS groups at the end of hydrophilic spacer arms and is used to couple ligands containing amino groups.

Technical Data
Spacer Arm Polymerized hydrophilic carbon chain
Porosity 1000Å
Average Particle Size 50um
Substitution Level 100-200 uEq/gm of NHS groups

Product list

Cat. Product Quantity Promo Price Order
NPHS-25 NuGel™ Poly-NHS 50 mL 470 € Order

HemoVoid™ – Hemoglobin Depletion From Erythrocytes

– Hemoglobin voids in flow-through >98%, with <30 minute bind/wash/elute protocol
– Hemoglobin removal from red cell lysates for RBC proteomics
– Hemoglobin removal from hemolyzed serum, blood and dried blood spot/blood card
– Enrichment of hemoglobin variants.
– Low abundance protein and enzyme enrichment
– Disposable, cost-effective and high-throughput
– Mild elution maintains tertiary structure and simple transfer to secondary analysis
– Removes hemoglobin from species including human, sheep, bovine, goat, etc.
– Removes hemoglobin from organs, tissues.
– The eluted fractions retain their enzymatic and biological activity
– The eluted fraction is compatible with LC-MS, activity based protein profiling and proteomic studies.

HemoVoid™ , a silica-based protein enrichment matrix, removes hemoglobin from erythrocyte lysate samples while concentrating low abundance, and/or low molecular weight proteins. The HemoVoid™ protocol uses mild buffers; the protocol conditions are so gentle that native enzyme activity is retained in elution fractions.

HemoVoid™ derives from a silica-based library of individual mixed-mode ligand combinations (ionic, hydrophobic, aromatic, polymer). The library was designed to facilitate weak binding of proteins, allowing for rapid elution from the matrix without any foreknowledge of the variety of proteins contained in the starting sample. HemoVoid™ depletes hemoglobin from red cell lysates while improving the resolution of less abundant blood proteins.

Product list:

Cat. Product Quantity Promo Price Order
HVK-05 HemoVoid™, Hemoglobin Depletion 5 mL 291 € Order
HVK-10 HemoVoid™, Hemoglobin Depletion 10 mL 382 € Order
HVK-50 HemoVoid™, Hemoglobin Depletion 50 mL 1300 € Order
HVK-100 HemoVoid™, Hemoglobin Depletion 100 mL 2029 € Order